- endocytosis |
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| Bibliography | |
| Endocytosis | ||
| Bibliography | ||
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Major papers and claim | |
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Recent papers | |
| Major papers and claim | ||
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1. COURTOY P.J. ; QUINTART J. and BAUDHUIN P. (1984). Shift of equilibrium density induced by 3,3'-diaminobenzidine cytochemistry : a new procedure for the analysis and purification of peroxidase-containing organelles. J. Cell Biol. 98:870-876 (IF2003 = 12.023) A two-step density-shift procedure was validated to purify predefined endocytic organelles, that can be simultaneously identified by utrastructural cytochemistry. This method produced the first highly purified endosomal fractions, established the molecular identity of endosomal compartments and demonstrated the sorting function of endosomes. |
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2. BARON R; NEFF L. ; LOUVARD D. and COURTOY P.J. (1985). Cell-mediated extracellular acidification and bone resorption. J. Cell Biol. 101:2210-2222 (IF2003 = 12.023) This paper presented the first experimental evidence that osteoclast actively acidify bone resorption lacunae by proton pumping, thereby challenging the model of acidic anion (lactate) release; it received > 400 citations. |
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3. CUPERS Ph. ; VEITHEN A. ; KISS A. ; BAUDHUIN P. and COURTOY P.J. (1994). Clathrin polymerization is not required for bulk-phase pinocytosis in rat foetal fibroblasts, J. Cell Biol. 127:725-735 (IF 2003 = 12.023) Using a combination of detailed kinetic and morphometric data at very short intervals of uptake, this paper established that bulk fluid-phase and membrane internalisation may proceed at a normal rate in the absence of clathrin polymerisation. This combined evidence quantitively supported the concept that although clathrin-coated pits are necessary for accelerated receptor-mediated endocytosis, clathrin polymerisation is not required for the basic endocytic machinery. This put a new perspective on clathrin-independent endocytosis and was rated as "of outstanding interest" in Current opinions in Cell Biology. |
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4. AMYERE M. ; PAYRASTRE B. ; KRAUSE U. ; VAN DER SMISSEN P. ; VEITHEN A. and COURTOY P.J. (2000) Constitutive macropinocytosis in oncogene-transformed fibroblasts depends on sequential permanent activation of phosphoinositide 3-kinase and phospholipase C. Mol. Biol. Cell 15:3453-3467 (IF 2003 = 7.454) Transient macropinocytosis is a key property for antigen presentation and is presumably subverted by enteropathogens for non-opsonized apical phagocytosis. This paper demonstrated that the two paradigmatic oncogenes, v-Src and Ras, trigger a constitutive macropinocytotic activity, via the sequential activation of phosphoinositide 3-kinase and phopholipase C. These two early enzymatic relays are generally regarded as independent or parallel in signalling cascades. |
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5. CHRISTENSEN E.I. ; DEVUYST O. ; DOM G. ; NIELSEN R. ; VAN DER SMISSEN P. ; VERROUST P. ; LERUTH M. ; GUGGINO W.B. and COURTOY P.J. (2003) Loss of chloride channel ClC-5 impairs endocytosis by defective trafficking of megalin and cubilin in kidney proximal tubules. Proc. Natl Acad Sci. USA 100:8472-8477 (IF 2003 = 10.272) Genetic inactivation of the chloride channel, ClC-5, that is preferentially expressed in endosomes of kidney proximal tubules, can explain some forms of hereditary kidney stones (Dent's disease). This paper accounted for an apical endocytic defect of ultrafiltrated low-molecular weight proteins in ClC-5 KO mice, by impaired trafficking of their multiligand tandem receptors, megalin and cubilin. This provided the first molecular explanation for a kidney Fanconi syndrome. |
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